49. Molecular biology applications in the practice of clinical laboratories: Difference between revisions
(Created page with "Molecular biology methods * High sensitivity and specificity * Expensive * Real-time PCR ** Visualization – fluorescent dyes, hybridization * DNA sequencing ** Next generation sequencing ** Whole exome sequencing * Benefit * Diagnosis ** Prognosis ** Therapy response * Monogenic disorders ** Sickle cell anaemia ** Thalassaemia * Microbiological assays ** HepC ** HepB ** CMV ** <abbr>EBV</abbr> ** HSV ** Gonorrhoea * Inherited conditions ** Factor V Leiden ** Prothr...") |
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Molecular biology methods | * Molecular biology methods | ||
** High sensitivity and specificity | |||
* High sensitivity and specificity | ** Expensive | ||
* Expensive | ** Real-time PCR | ||
* Real-time PCR | *** Visualization – fluorescent dyes, hybridization | ||
** Visualization – fluorescent dyes, hybridization | ** DNA sequencing | ||
* DNA sequencing | *** Next generation sequencing | ||
** Next generation sequencing | *** Whole exome sequencing | ||
** Whole exome sequencing | |||
* Benefit | * Benefit | ||
* Diagnosis | ** Diagnosis | ||
** Prognosis | ** Prognosis | ||
** Therapy response | ** Therapy response | ||
| Line 38: | Line 36: | ||
** CYP2C9, VKORC1 – warfarin | ** CYP2C9, VKORC1 – warfarin | ||
** TPMT – azathioprine | ** TPMT – azathioprine | ||
[[Category:Clinical Biochemistry (POTE course)]] | |||
Latest revision as of 11:05, 18 June 2024
- Molecular biology methods
- High sensitivity and specificity
- Expensive
- Real-time PCR
- Visualization – fluorescent dyes, hybridization
- DNA sequencing
- Next generation sequencing
- Whole exome sequencing
- Benefit
- Diagnosis
- Prognosis
- Therapy response
- Monogenic disorders
- Sickle cell anaemia
- Thalassaemia
- Microbiological assays
- HepC
- HepB
- CMV
- EBV
- HSV
- Gonorrhoea
- Inherited conditions
- Factor V Leiden
- Prothrombin mutation
- Gilbert syndrome (UGT)
- Congenital metabolic disorders
- NF1
- CFTR
- Neurodegenerative diseases
- Pharmacogenomics
- Polymorphisms in Pgp
- CYP2C19 – clopidogrel
- CYP2C9, VKORC1 – warfarin
- TPMT – azathioprine