49. Molecular biology applications in the practice of clinical laboratories: Difference between revisions

From greek.doctor
(Created page with "Molecular biology methods * High sensitivity and specificity * Expensive * Real-time PCR ** Visualization – fluorescent dyes, hybridization * DNA sequencing ** Next generation sequencing ** Whole exome sequencing * Benefit * Diagnosis ** Prognosis ** Therapy response * Monogenic disorders ** Sickle cell anaemia ** Thalassaemia * Microbiological assays ** HepC ** HepB ** CMV ** <abbr>EBV</abbr> ** HSV ** Gonorrhoea * Inherited conditions ** Factor V Leiden ** Prothr...")
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Revision as of 11:04, 18 June 2024

Molecular biology methods

  • High sensitivity and specificity
  • Expensive
  • Real-time PCR
    • Visualization – fluorescent dyes, hybridization
  • DNA sequencing
    • Next generation sequencing
    • Whole exome sequencing
  • Benefit
  • Diagnosis
    • Prognosis
    • Therapy response
  • Monogenic disorders
    • Sickle cell anaemia
    • Thalassaemia
  • Microbiological assays
    • HepC
    • HepB
    • CMV
    • EBV
    • HSV
    • Gonorrhoea
  • Inherited conditions
    • Factor V Leiden
    • Prothrombin mutation
    • Gilbert syndrome (UGT)
    • Congenital metabolic disorders
    • NF1
    • CFTR
    • Neurodegenerative diseases
  • Pharmacogenomics
    • Polymorphisms in Pgp
    • CYP2C19 – clopidogrel
    • CYP2C9, VKORC1 – warfarin
    • TPMT – azathioprine