7. HBs antigen positivism (Shikata orcein): Difference between revisions
(Created page with "'''Staining''': Shikata orcein '''Organ''': Liver '''Description''': The liver parenchyme in this slide has been divided into “pseudolobules”. These pseudolobules don’t correspond to the normal liver lobules and have wildly varying sizes. The pseudolobules are surrounded by scar tissue. In two of the pseudolobules there are hepatocytes which are HBsAG positive. Their morphology is called “ground-glass hepatocytes”. '''Diagnosis''': HBs antigen positive chr...") |
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'''Staining''': Shikata orcein | [[File:HBs antigen positivity - overview.png|thumb|Overview|360x360px]]'''Staining''': Shikata orcein | ||
'''Organ''': Liver | '''Organ''': Liver | ||
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* Hepatitis B infection | * Hepatitis B infection | ||
[[File:HBs antigen positivity - hepatocytes.png|thumb|This shows the HBsAg positive hepatocytes|365x365px]]'''Theory''': | |||
'''Theory''': | |||
Normal orcein stains elastic fibres while Shikata orcein stains the hepatitis B surface antigen (HBsAg) as well. This staining is not used in clinical practice as serology is preferred instead. | Normal orcein stains elastic fibres while Shikata orcein stains the hepatitis B surface antigen (HBsAg) as well. This staining is not used in clinical practice as serology is preferred instead. | ||
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The extensive pseudolobule formation with scar tissue surrounding them is characteristic for fully mature cirrhosis. | The extensive pseudolobule formation with scar tissue surrounding them is characteristic for fully mature cirrhosis. | ||
[[Category:Pathology 2 - Histopathology slides]] | [[Category:Pathology 2 - Histopathology slides]] | ||
Latest revision as of 14:25, 7 July 2024
Staining: Shikata orcein
Organ: Liver
Description:
The liver parenchyme in this slide has been divided into “pseudolobules”. These pseudolobules don’t correspond to the normal liver lobules and have wildly varying sizes. The pseudolobules are surrounded by scar tissue.
In two of the pseudolobules there are hepatocytes which are HBsAG positive. Their morphology is called “ground-glass hepatocytes”.
Diagnosis: HBs antigen positive chronic hepatitis with cirrhosis
Causes:
- Hepatitis B infection
Theory:
Normal orcein stains elastic fibres while Shikata orcein stains the hepatitis B surface antigen (HBsAg) as well. This staining is not used in clinical practice as serology is preferred instead.
Because hepatocytes are HBsAg positive (ground- glass morphology) can we be certain that this chronic hepatitis was caused by Hep B infection.
The extensive pseudolobule formation with scar tissue surrounding them is characteristic for fully mature cirrhosis.