Plasma protein: Difference between revisions
(Created page with "{{:Stub}} <section begin="clinical biochemistry" /> * Total protein ** Biuret test (copper forms purple complex with proteins in alkaline solution) ** Colour intensity proportional to protein concentration – photometry * Albumin ** Albumin forms green complex with BCG at pH 4,1 ** Photometry * Specific proteins ** Measurement based on immunogenicity – use of monoclonal or polyclonal antibodies ** Immunoassay – biochemical test measuring concentration of protein b...") |
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<section begin="clinical biochemistry" /> | <section begin="clinical biochemistry" /> | ||
* Total protein | * Total protein | ||
** Normal serum level 66 - 83 g/L | |||
** Normal urine level < 0,1 g/L | |||
** Biuret test (copper forms purple complex with proteins in alkaline solution) | ** Biuret test (copper forms purple complex with proteins in alkaline solution) | ||
** Colour intensity proportional to protein concentration – photometry | ** Colour intensity proportional to protein concentration – photometry | ||
* Albumin | * Albumin | ||
** Normal serum level 35 - 53 g/L | |||
** Albumin forms green complex with BCG at pH 4,1 | ** Albumin forms green complex with BCG at pH 4,1 | ||
** Photometry | ** Photometry | ||
Revision as of 21:44, 21 March 2024
This article is a stub, meaning that it is unfinished. It will eventually be expanded.
- Total protein
- Normal serum level 66 - 83 g/L
- Normal urine level < 0,1 g/L
- Biuret test (copper forms purple complex with proteins in alkaline solution)
- Colour intensity proportional to protein concentration – photometry
- Albumin
- Normal serum level 35 - 53 g/L
- Albumin forms green complex with BCG at pH 4,1
- Photometry
- Specific proteins
- Measurement based on immunogenicity – use of monoclonal or polyclonal antibodies
- Immunoassay – biochemical test measuring concentration of protein by using antibody
- Enzyme immunoassay (EIA)
- Enzyme-linked immunosorbent assay (ELISA)
- Radioimmunoassay (RIA)
- Fluorescence
- Proteins
- Microalbumin (high sensitivity albumin measurement)
- Hormones
- Tumour markers
- Drugs
- B12
- Folic acid
- Transferrin
- Ferritin
- EPO
- HCG
- Troponin
- Procalcitonin
- Separation techniques
- Chromatography
- Electrophoresis – sample added to gel -> electric field applied -> molecules separated by electric charge -> stained to make charged molecules visible
- Qualitative, semi-quantitative!
- Serum, not plasma (fibrinogen forms a band)
- Serum electrophoresis – monoclonal paraproteins
- CSF electrophoresis – multiple sclerosis
- Haemoglobin electrophoresis – haemoglobinopathies
- Lipid electrophoresis – dyslipidaemias
- Urine electrophoresis – Bence-Jones protein