8. Steatosis hepatis: Difference between revisions
(Created page with "'''Staining''': HE and oil-red '''Organ''': Liver '''Description''': HE: We can see the portal triad, meaning that we are looking at a liver. There is panlobular fat accumulation (fat is present in the whole lobule) in the whole slide. Oil-red: With this staining the fat is reddish-orangeish. '''Diagnosis''': Hepatic steatosis Causes: * Alcohol consumption * Metabolic syndrome (DM2, obesity) * Medications '''Theory''': When fixating the normal slides, we use for...") |
No edit summary |
||
| Line 1: | Line 1: | ||
'''Staining''': HE and oil-red | [[File:Hepatic steatosis HE overview.jpg|thumb|Overview of HE]]'''Staining''': HE and oil-red | ||
'''Organ''': Liver | '''Organ''': Liver | ||
| Line 15: | Line 15: | ||
* Alcohol consumption | * Alcohol consumption | ||
* Metabolic syndrome (DM2, obesity) | * Metabolic syndrome (DM2, obesity) | ||
* Medications | * Medications[[File:Hepatic steatosis oil red overview.jpg|thumb|Overview of oil red]] | ||
'''Theory''': | '''Theory''': | ||
| Line 26: | Line 26: | ||
The enzyme ADH that metabolizes ethanol depletes NAD+ and increases NADH. NADH stimulates fatty acid synthesis. NAD+ is needed for β-oxidation of the fat. This causes fat to accumulate in the hepatocytes. | The enzyme ADH that metabolizes ethanol depletes NAD+ and increases NADH. NADH stimulates fatty acid synthesis. NAD+ is needed for β-oxidation of the fat. This causes fat to accumulate in the hepatocytes. | ||
[[File:Hepatic steatosis HE portal triad.jpg|thumb|A portal triad, with the artery, bile duct and vein in that order, clockwise.|left]] | |||
[[File:Hepatic steatosis HE portal triad.jpg | |||
[[File:Hepatic steatosis HE panlobar fat.jpg|center|thumb|Panlobular fat accumulation.]] | [[File:Hepatic steatosis HE panlobar fat.jpg|center|thumb|Panlobular fat accumulation.]] | ||
[[File:Hepatic steatosis HE bile.jpg | [[File:Hepatic steatosis HE bile.jpg|thumb|Bile between cells.|left]] | ||
[[File:Hepatic steatosis oil red fat.jpg|center|thumb|Fat in hepatocytes as seen in oil-red staining. The big orange blobs are fat.]] | [[File:Hepatic steatosis oil red fat.jpg|center|thumb|Fat in hepatocytes as seen in oil-red staining. The big orange blobs are fat.]] | ||
[[Category:Pathology 1 - Histopathology slides]] | [[Category:Pathology 1 - Histopathology slides]] | ||
Latest revision as of 11:14, 5 July 2024
Staining: HE and oil-red
Organ: Liver
Description:
HE: We can see the portal triad, meaning that we are looking at a liver. There is panlobular fat accumulation (fat is present in the whole lobule) in the whole slide.
Oil-red: With this staining the fat is reddish-orangeish.
Diagnosis: Hepatic steatosis
Causes:
- Alcohol consumption
- Metabolic syndrome (DM2, obesity)
- Medications
Overview of oil red
Theory:
When fixating the normal slides, we use formalin. Formalin dissolves fat, which is why fat isn’t directly seen in the HE slides. In the oil-red slide there was no formalin but instead the slide was frozen. This preserves the fat.
Oil-red can be used to determine the amount of fat and therefore the severity of the steatosis.
In this slide there was panlobular fat, but in some cases there can be pericentral fat, where fat can only be found around the central veins.
The enzyme ADH that metabolizes ethanol depletes NAD+ and increases NADH. NADH stimulates fatty acid synthesis. NAD+ is needed for β-oxidation of the fat. This causes fat to accumulate in the hepatocytes.
