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8. Steatosis hepatis: Difference between revisions
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(Created page with "'''Staining''': HE and oil-red '''Organ''': Liver '''Description''': HE: We can see the portal triad, meaning that we are looking at a liver. There is panlobular fat accumulation (fat is present in the whole lobule) in the whole slide. Oil-red: With this staining the fat is reddish-orangeish. '''Diagnosis''': Hepatic steatosis Causes: * Alcohol consumption * Metabolic syndrome (DM2, obesity) * Medications '''Theory''': When fixating the normal slides, we use for...") |
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'''Staining''': HE and oil-red | [[File:Hepatic steatosis HE overview.jpg|thumb|Overview of HE]]'''Staining''': HE and oil-red | ||
'''Organ''': Liver | '''Organ''': Liver | ||
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* Alcohol consumption | * Alcohol consumption | ||
* Metabolic syndrome (DM2, obesity) | * Metabolic syndrome (DM2, obesity) | ||
* Medications | * Medications[[File:Hepatic steatosis oil red overview.jpg|thumb|Overview of oil red]] | ||
'''Theory''': | '''Theory''': | ||
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The enzyme ADH that metabolizes ethanol depletes NAD+ and increases NADH. NADH stimulates fatty acid synthesis. NAD+ is needed for β-oxidation of the fat. This causes fat to accumulate in the hepatocytes. | The enzyme ADH that metabolizes ethanol depletes NAD+ and increases NADH. NADH stimulates fatty acid synthesis. NAD+ is needed for β-oxidation of the fat. This causes fat to accumulate in the hepatocytes. | ||
[[File:Hepatic steatosis HE portal triad.jpg|thumb|A portal triad, with the artery, bile duct and vein in that order, clockwise.|left]] | |||
[[File:Hepatic steatosis HE portal triad.jpg | |||
[[File:Hepatic steatosis HE panlobar fat.jpg|center|thumb|Panlobular fat accumulation.]] | [[File:Hepatic steatosis HE panlobar fat.jpg|center|thumb|Panlobular fat accumulation.]] | ||
[[File:Hepatic steatosis HE bile.jpg | [[File:Hepatic steatosis HE bile.jpg|thumb|Bile between cells.|left]] | ||
[[File:Hepatic steatosis oil red fat.jpg|center|thumb|Fat in hepatocytes as seen in oil-red staining. The big orange blobs are fat.]] | [[File:Hepatic steatosis oil red fat.jpg|center|thumb|Fat in hepatocytes as seen in oil-red staining. The big orange blobs are fat.]] | ||
[[Category:Pathology 1 - Histopathology slides]] | [[Category:Pathology 1 - Histopathology slides]] | ||